| 1. Netherland Inn | 2a. Bloomingdale Road | 2b. Fort Patrick |
| 3. Anderson Blockhouse | 4. Moccasin Gap | 5. Gate City Courthouse |
| 7. Speer's Ferry | 8. Stock Creek | 9a. Natural Tunnel |
| 9b. Blockhouse | 10. Duffield | 11. Powell Mountain Overlook |
| 12. James Boone Massacre | 13. DOT Intersection | 14. Powell River Bridge |
| 15. Confedrate Cemetery | 16. Dr. Still's Birthplace | 17. Natural Bridge |
| 18. East of Flatwoods | 19. Wilson Hill | 20. Silver Leaf |
| 21. West of Ewing | 22. East End of Bike Trail | 23. Wilderness Road State Park |
| 24. Cumberland Gap |